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Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation ofmembrane proteins

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1188-2

摘要： Explaintheadsorption, uptake and transmembrane transport of PAHs by bacteria. Analyze functional regulation of membrane proteins inthe transmembrane transport. Proteomics technology such as iTRAQ labeling was used to access expressed proteins. Single cell analysis technology wereused to study the morphological structure. In recent years, increasing research has been conducted on transmembrane transport processes and the mechanisms behind the microbial breakdown of polycyclic aromatic hydrocarbons (PAHs), including the role of membrane proteins in transmembrane transport and the mode of transmission. This article explains the adsorption, uptake and transmembrane transport of PAHs by bacteria, the regulation of membrane protein function during the transmembrane transport. There are three different regulation mechanisms for uptake, depending on the state and size of the oil droplets relative to the size of the microbial cells, which are (i) direct adhesion, (ii) emulsification and pseudosolubilization, and (iii) interfacial uptake. Furthermore, two main transmembrane transport modes are introduced, which are (i) active transport and (ii) passive uptake and active efflux mechanism. Meanwhile, introduce the proteomics and single cell analysis technology used to address these areas of research, such as Isobaric tags for relative and absolute quantitation (iTRAQ) technology and Nano Secondary ion mass spectrometry (Nano-SIMS). Additionally, analyze the changes in morphology and structure and the characteristics of microbial cell membranes in the process of transmembrane transport. Finally, recognize the microscopic mechanism of PAHs biodegradation in terms of cell and membrane proteins are of great theoretical and practical significance for understanding the factors that influence the efficient degradation of PAHs contaminants in soil and for remediating the PAHs contamination in this area with biotechnology.

关键词： Polycyclic aromatic hydrocarbons     Transmembrane transport     Adsorption and uptake of hydrocarbons     Proteomics     Functional regulation of membrane protein     Single cell analysis technology    

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Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

《环境科学与工程前沿（英文）》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0855-9

摘要： pH values of the BSA solution significantly impact the process of membrane fouling. Dramatic flux decline is caused by membrane–BSA adhesion force at start of filtration. XDLVO theory shows the polar or Lewis acid–base interaction plays a major role in membrane fouling. To further determine the fouling behavior of bovine serum albumin (BSA) on different hydrophilic PVDF ultrafiltration (UF) membranes over a range of pH values, self-made atomic force microscopy (AFM) colloidal probes were used to detect the adhesion forces of membrane–BSA and BSA–BSA, respectively. Results showed that the membrane–BSA adhesion interaction was stronger than the BSA–BSA adhesion interaction, and the adhesion force between BSA–BSA-fouled PVDF/PVA membranes was similar to that between BSA–BSA-fouled PVDF/PVP membranes, which indicated that the fouling was mainly caused by the adhesion interaction between membrane and BSA. At the same pH condition, the PVDF/PVA membrane–BSA adhesion force was smaller than that of PVDF/PVP membrane–BSA, which illustrated that the more hydrophilic the membrane was, the better antifouling ability it had. The extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) theory predicts that the polar or Lewis acid–base (AB) interaction played a dominant role in the interfacial free energy of membrane–BSA and BSA–BSA that can be affected by pH. For the same membrane, the pH values of a BSA solution can have a significant impact on the process of membrane fouling by changing the AB component of free energy.

关键词： PVDF membrane     Membrane fouling     Adhesion force     Protein     Interfacial free energy    

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Mechanism of ethanol/water reverse separation through a functional graphene membrane: a molecular simulation

《化学科学与工程前沿（英文）》 2023年 第17卷 第3期   页码 347-357 doi: 10.1007/s11705-022-2246-z

摘要： Reverse-selective membranes have attracted considerable interest for bioethanol production. However, to date, the reverse-separation performance of ethanol/water is poor and the separation mechanism is unclear. Graphene-based membranes with tunable apertures and functional groups have shown substantial potential for use in molecular separation. Using molecular dynamics simulations, for the first time, we reveal two-way selectivity in ethanol/water separation through functional graphene membranes. Pristine graphene (PG) exhibits reverse-selective behavior with higher ethanol fluxes than water, resulting from the preferential adsorption for ethanol. Color flow mappings show that this ethanol-permselective process is initiated by the presence of ethanol-enriched and water-barren pores; this has not been reported in previous studies. In contrast, water molecules are preferred for hydroxylated graphene membranes because of the synergistic effects of molecular sieving and functional-group attraction. A simulation of the operando condition shows that the PG membrane with an aperture size of 3.8 Å achieves good separation performance, with an ethanol/water separation factor of 34 and a flux value of 69.3 kg∙m‒2∙h‒1∙bar‒1. This study provides new insights into the reverse-selective mechanism of porous graphene membranes and a new avenue for efficient biofuel production.

关键词： reverse separation     graphene membrane     ethanol/water separation     molecular simulation    

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Charge and separation characteristics of nanofiltration membrane embracing dissociated functional groups

Zhun MA,Meng WANG,Xueli GAO,Congjie GAO

《环境科学与工程前沿（英文）》 2014年 第8卷 第5期   页码 650-658 doi: 10.1007/s11783-013-0605-1

摘要： The current work focused on the investigation of charge and separation characteristics of nanofiltration (NF) membrane embracing dissociated functional groups under different electrolyte solutions. The electro-kinetic method was carried out to assess the membrane volume charge density ( ) with different salt concentrations ranging from 0.1 to 10 mol·m and different electrolyte species, such as type 1–1, type 2–1 and type 3–1. The Donnan steric pore model-dielectric exclusion (DSPM-DE) model was employed to evaluate the separation characteristics of the NF membrane for wide range of electrolyte concentration (from 25.7 to 598.9 mol·m ). The results indicated that the dissociation of the hydrophilic functional groups and the specific adsorption contributed to charge formation on membrane surface. The former played a dominant role in type 1–1 and type 2–1 electrolytes at dilute aqueous solutions (0.1–0.5 mol·m ). However, for type 3–1 electrolyte, specific adsorption should contribute to the charge effect to a large extent. Moreover, the correlation between the volume charge density and feed concentration was in accordance with Freundlich isotherm. Furthermore, it was found that the separation characteristic of NF membrane could be evaluated well by DSPM-DE model coupling with electro-kinetic method in a whole concentration range.

关键词： Sulfonated polyethersulfone nanofiltration membrane     charge characteristics     electro-kinetic method     volume charge density     separation behavior    

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Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine

Jianqing PAN PhD, Qin ZHANG MD, Daowen WANG MD, PhD,

《医学前沿（英文）》 2009年 第3卷 第4期   页码 390-395 doi: 10.1007/s11684-009-0087-5

摘要： We constructed a eukaryotic expression plasmid encoding Epstein-Barr virus latent membrane protein 2 (EBV, LMP2) and evaluated its effects on humoral immunity. First, the encoding sequence of the EBV was amplified from B95−8 cell RNA by reverse transcription polymerase chain reaction (RT-PCR) and then was directionally cloned into eukaryotic expression vector pcDNA3.1. It was employed to evaluate immune response of the mice inoculated doubly with the DNA vaccine. The serum antibody against LMP2 was detected with enzyme-linked immunosorbent assay (ELISA). The recombinant plasmid pcDNA3.1- was confirmed by the restrictive endonuclease analysis and sequence analysis. The serum titer of IgG antibody against LMP2 epitope in the mice immunized with the DNA vaccine encoding LMP2 was up to 1∶4000. In conclusion, the EBV DNA vaccine can induce a strong humoral immune response in mice.

关键词： Epstein-Barr virus     latent membrane protein 2     nasopharyngeal carcinoma     humoral immunity    

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Topological reorganization and functional alteration of distinct genomic components in gallbladder cancer

《医学前沿（英文）》 doi: 10.1007/s11684-023-1008-8

摘要： Altered three-dimensional architecture of chromatin influences various genomic regulators and subsequent gene expression in human cancer. However, knowledge of the topological rearrangement of genomic hierarchical layers in cancer is largely limited. Here, by taking advantage of in situ Hi-C, RNA-sequencing, and chromatin immunoprecipitation sequencing (ChIP-seq), we investigated structural reorganization and functional changes in chromosomal compartments, topologically associated domains (TADs), and CCCTC binding factor (CTCF)-mediated loops in gallbladder cancer (GBC) tissues and cell lines. We observed that the chromosomal compartment A/B switch was correlated with CTCF binding levels and gene expression changes. Increased inter-TAD interactions with weaker TAD boundaries were identified in cancer cell lines relative to normal controls. Furthermore, the chromatin short loops and cancer unique loops associated with chromatin remodeling and epithelial–mesenchymal transition activation were enriched in cancer compared with their control counterparts. Cancer-specific enhancer–promoter loops, which contain multiple transcription factor binding motifs, acted as a central element to regulate aberrant gene expression. Depletion of individual enhancers in each loop anchor that connects with promoters led to the inhibition of their corresponding gene expressions. Collectively, our data offer the landscape of hierarchical layers of cancer genome and functional alterations that contribute to the development of GBC.

关键词： 3D genome     cancer     TADs     loop     gene regulation    

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Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor

GONG Xiaowei, WEI Jie, LI Yusheng, CHENG Weiwei, DENG Peng, JIANG Yong

《医学前沿（英文）》 2007年 第1卷 第3期   页码 248-252 doi: 10.1007/s11684-007-0047-x

摘要： The aim of this study was to investigate the role of p38 mitogen-activated protein kinase (MAPK) in cell migration induced by platelet-derived growth factor (PDGF). Western blot was performed to detect the phosphorylation of p38 in NIH3T3 cells treated with PDGF. A Transwell cell migration system was used to determine the effects of PDGF treatment on the migration of NIH3T3 cells and the influence of deficiency on this process in a gene knockout (p38) mouse embryonic fibroblast cell line. On the stimulation of PDGF, the migration of NIH3T3 cells was significantly increased (〈0.001) compared to the control and p38 MAP kinase was simultaneously phosphorylated. Furthermore, the PDGF-induced cell migration was significantly blocked in gene knockout (p38) mouse embryonic fibroblasts (MEFs) (〈0.001) as compared with the wild type cells (p38). p38 MAPK plays an important role in the regulation of cell migration induced by PDGF.

关键词： control     stimulation     mitogen-activated     growth factor     process    

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Regulation of radicals by hydrogen-donor solvent in direct coal liquefaction

《化学科学与工程前沿（英文）》 2022年 第16卷 第12期   页码 1689-1699 doi: 10.1007/s11705-022-2186-7

摘要： Radicals are important intermediates in direct coal liquefaction. Certain radicals can cause the cleavage of chemical bonds. At high temperatures, radical fragments can be produced by the splitting of large organic molecules, which can break strong chemical bonds through the induction pyrolysis of radicals. The reaction between the formation and annihilation of coal radical fragments and the effect of hydrogen-donor solvents on the radical fragments are discussed in lignite hydrogenolysis. Using the hydroxyl and ether bonds as indicators, the effects of different radicals on the cleavage of chemical bond were investigated employing density functional theory calculations and lignite hydrogenolysis experiments. Results showed that the adjustment of the coal radical fragments could be made by the addition of hydrogen-donor solvents. Results showed that the transition from coal radical fragment to H radical leads to the variation of product distribution. The synergistic mechanism of hydrogen supply and hydrogenolysis of hydrogen-donor solvent was proposed.

关键词： direct coal liquefaction     hydrogen-donor solvent     induced pyrolysis     radical mechanism     density functional theory calculations    

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Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

《医学前沿（英文）》 2008年 第2卷 第3期   页码 235-238 doi: 10.1007/s11684-008-0044-8

摘要： The aim of this study is to investigate the effect of tyrosine kinase Src on Tyrosine 307(Y307) phosphorylation, protein phosphatase 2A (PP2A) activity, and on tau phosphorylation. Specific Src siRNA was transfected into cultured mouse neuroblastoma N2a cells to inhibit the expression of Src protein, and the phosphorylation levels of PP2A Y307 and tau at different sites, as well as PP2A activity were detected at different time points after siRNA transfection. Twelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreased PP2A Y307 phosphorylation. However, the total PP2A protein level was also decreased, together with a decreased PP2A activity. Tau was hyperphosphorylated at the Ser198/199/202 sites. Multiple factors may be involved in the cellular regulation of PP2A activity. Inhibiting Src expression could induce inactivation of PP2A and tau hyperphosphorylation.

关键词： hyperphosphorylation     PP2A activity     cellular regulation     siRNA     siRNA transfection    

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Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 97-104 doi: 10.15302/J-FASE-2018248

摘要：

Plant nucleotide binding domain and leucine-rich repeat (NLR) receptors recognize pathogen effectors directly or indirectly and mediate innate immune responses. NLR-mediated immunity also has direct impacts on plant growth and development, as well as yield and survival. The levels of NLR proteins are therefore intricately controlled in plants to balance defense responses and other processes. In recent years, the ubiquitination-26S proteasome system and the HSP90 chaperones have emerged as having key functions in the regulation of NLR stability. The N-end rule pathway of protein degradation is also directly linked to NLR stability. Recent progress in the regulation of NLR stability and turnover is summarized here, focusing on the key components and pathways.

关键词： E3 ubiquitin ligase     degradation     nucleotide-binding leucine-rich repeat receptor     plant immunity     proteasome     protein stability     ubiquitination    

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Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 327-335 doi: 10.1007/s11783-014-0763-9

摘要： A novel method was applied to co-recover proteins and humic acid from the dewatered sewage sludge for liquid fertilizer and animal feed. The proteins in sewage sludge were first extracted using the processes of ultra-sonication and acid precipitation, and then the humic acid was recovered via membrane filtration. The extraction efficiency was 125.9 mg humic acid?g VSS volatile suspended solids (VSS) and 123.9 mg proteins?g VSS at the optimal ultrasonic density of 1.5 W?mL . FT-IR spectrum results indicated that the recovered proteins and humic acid showed similar chemical characteristic to the natural proteins and humic acid. The acidic solution (pH 2) could be recycled and used more than 10 times during the co-recovery processes. In addition, the dewatered sludge could be easily biodegraded when the humic acid and proteins are extracted, which was essential for further utilization. These findings are of great significance for recovering valuable nutrient from sewage sludge.

关键词： sewage sludge     co-recovery     proteins     humic acid     recycling     biodegradation rate    

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靶向膜蛋白的抗体药物开发的新进展 Review

Georgina To’a Salazar, 黄子逸, 张凝艳, 张学光, 安志强

《工程（英文）》 2021年 第7卷 第11期   页码 1541-1551 doi: 10.1016/j.eng.2020.11.013

摘要：

在疾病干预的众多膜蛋白靶标中，G蛋白偶联受体（GPCR）作为人体内最大的膜受体蛋白家族，成为很多药物的重要靶点，其次是离子通道、转运蛋白和激酶等。膜蛋白在细胞信号转导和运输中发挥了关键作用，当前药物研发面临的挑战在于进一步发掘此类膜蛋白的潜在靶点的干预价值，开发治疗性抗体药物。鉴于特异性抗体能够识别膜蛋白的灵敏特性，以及随着基因工程技术的进步，对已有抗体进行加工改造可获得适应多个靶点蛋白的特异性抗体。然而，成功分离特异靶向膜蛋白抗体取决于一系列因素。我们更易研制和识别结构简单且具有长片段胞外区的抗体分子，但对于高难度的靶点蛋白，如GPCR和其他复杂膜蛋白往往难以得到具有活性的候选抗体。目前若要开发针对复杂膜蛋白（如GPCR、离子通道、转运蛋白和激酶）的抗体药物，必须从抗原靶点设计、抗体筛选策略、先导抗体优化及药物研发模式方面进行考虑。深入研究靶标膜蛋白的结构有助于推进治疗性抗体药物的开发进程。本文概述了抗体靶向复杂膜蛋白的优势和挑战，以及膜蛋白抗原制备和抗体研发策略的最新进展。

关键词： 抗体治疗     复杂膜蛋白     离子通道     转运蛋白     膜结合酶     G蛋白偶联受体     药物发现    

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Moving policy and regulation forward for single-use plastic alternatives

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-021-1423-5

摘要： Single-use plastics are often used once or cannot be reused for extended periods. They are widely consumed with the rapid development of social economy. The waste generated by single-use plastics threatens ecosystem health by entering the environment and ultimately restricts sustainable human development. The innovation of sustainable and environmentally friendly single-use plastic alternative materials and the joint participation of governments, enterprises and the public are promising technologies and management approaches that can solve the problem of single-use plastics wastes. The development of single-use plastic alternative products can be promoted fundamentally only by improving relevant legislation and standards, providing differentiated industrial policies, encouraging scientific and technological innovation and expanding public participation.

关键词： Single-use plastic alternatives     Policy     Regulation     Sustainable development    

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Phase transition regulation and caloric effect

《能源前沿（英文）》 2023年 第17卷 第4期   页码 463-477 doi: 10.1007/s11708-023-0860-1

摘要： Solid state refrigeration based on caloric effect is regarded as a potential candidate for replacing vapor-compression refrigeration. Numerous methods have been proposed to optimize the refrigeration properties of caloric materials, of which single field tuning as a relatively simple way has been systemically studied. However, single field tuning with few tunable parameters usually obtains an excellent performance in one specific aspect at the cost of worsening the performance in other aspects, like attaining a large caloric effect with narrowing the transition temperature range and introducing hysteresis. Because of the shortcomings of the caloric effect driven by a single field, multifield tuning on multicaloric materials that have a coupling between different ferro-orders came into view. This review mainly focuses on recent studies that apply this method to improve the cooling performance of materials, consisting of enlarging caloric effects, reducing hysteresis losses, adjusting transition temperatures, and widening transition temperature spans, which indicate that further progress can be made in the application of this method. Furthermore, research on the sign of lattice and spin contributions to the magnetocaloric effect found new phonon evolution mechanisms, calling for more attention on multicaloric effects. Other progress including improving cyclability of FeRh alloys by introducing second phases and realizing a large reversible barocaloric effect by hybridizing carbon chains and inorganic groups is described in brief.

关键词： phase transition regulation     caloric effect     solid state refrigeration    

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标题 作者 时间 类型 操作

Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation ofmembrane proteins

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

期刊论文

Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

期刊论文

Mechanism of ethanol/water reverse separation through a functional graphene membrane: a molecular simulation

期刊论文

Charge and separation characteristics of nanofiltration membrane embracing dissociated functional groups

Zhun MA,Meng WANG,Xueli GAO,Congjie GAO

期刊论文

Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine

Jianqing PAN PhD, Qin ZHANG MD, Daowen WANG MD, PhD,

期刊论文

Topological reorganization and functional alteration of distinct genomic components in gallbladder cancer

期刊论文

Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor

GONG Xiaowei, WEI Jie, LI Yusheng, CHENG Weiwei, DENG Peng, JIANG Yong

期刊论文

Regulation of radicals by hydrogen-donor solvent in direct coal liquefaction

期刊论文

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

期刊论文

Regulation of NLR stability in plant immunity

Tao WANG, Jiaxin LI, Qian-Hua SHEN

期刊论文

Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

期刊论文

姜晓滨：膜结晶过程调控与强化（2022年4月16日）

2022年06月09日

会议视频

靶向膜蛋白的抗体药物开发的新进展

Georgina To’a Salazar, 黄子逸, 张凝艳, 张学光, 安志强

期刊论文

Moving policy and regulation forward for single-use plastic alternatives

期刊论文

Phase transition regulation and caloric effect

期刊论文